Tomas Strucko

The key plasmids from our Pichia tools paper academic.oup.com/femsyr/article… are now available @Addgene addgene.org/Uffe_Mortensen/ please share the news 🧑‍🔬🧬

Excited: We are Project 11 in King's Prize Doctoral Programme – Genome Data Science & AI for Therapeutic Target Discovery @KingsCollegeLon! Genetics + multi-omics + AI → new drug targets. Fully funded 4-yr PhD, starts Sept 2026. Deadline 28 Feb! Apply: kcl.ac.uk/research/targe…

New tool for filamentous fungi by @UHMortensenLab doi.org/10.1093/nar/gk… "RoCi—a single-step multi-copy integration system based on rolling-circle replication" 👇🍄🔧⚙️

Applications for @KingsCollegeLon @The_MRC Doctoral Training Partnership in Biomedical Sciences 2026 entry will open on the 8th October. Visit our website now to start preparing your application! kcl-mrcdtp.com/apply/applicat… #MRCDTP26 #PhD #London #Studentships

The PhD programme at King’s College London is now open — and everyone is encouraged to apply! Our group focuses on AI-driven synthetic biology, combining machine learning and experimental biology to engineer gene expression and design new proteins. If you’re interested in AI, systems biology, or engineering biology, this is a great place to grow and make an impact.

Latest from @UHMortensenLab …robialcellfactories.biomedcentral.com/articles/10.11…

I got hit by some rather sudden and extreme financial hardship so if anyone is in need of remote wetlab contract research, strictly BSL1, do let me know. Currently scrambling for gigs. Plant, Bacterial, Archaeal Bioeng Custom Lab Hardware Turn Key Genetic Design Please RT 💚

I am very excited to announce the publication of this new research article on "Orally delivered toxin–binding protein protects against diarrhoea in a murine cholera model", which is a topic I sincerely care about: nature.com/articles/s4146… Thanks to all co-authors and funders!

Excited to share a new research article from the @CAT4Science at @DTUbioengineer and great collaborators, which has just has been published in @Nature ! De novo designed proteins neutralize lethal snake venom toxins Read article here: nature.com/articles/s4158… @TimothyPJenkins

Do you want to automate strain design of your streptomycetes strains? Then check out StreptoCAD! Many thanks to @chriiswh, Søren Dalsgård Petersen, @kaiblin, @tilmweber and Rasmus John Normand Frandsen Find the preprint here: biorxiv.org/content/10.110…

@ATinyGreenCell @AkosNyerges 16 years from plate to plate = an impressive laboratory evolution experiment 😉

@AkosNyerges Oooh great point! Do you happen to know if the NEB branded BL21(DE3) carries that prophage as well? Mine is that line, only passaged over the last 16 years plate to plate bc I lack a -80C freezer. :)

Results from the first test run of my EasyBake Mutagenesis Oven: 0 sec - lawn 30sec - 82 colonies 60sec - 11 colonies 120sec - 10 colonies 240sec - 2 colonies Now to grow up some BL21 cells, make LB with X-Gal, and count LacZ mutants per CFU per second of UVC exposure :) 🔦🦠☠️

tidyplots Time to say goodbye to ggplot2?🫡 "a significant reduction of code complexity" vs ggplot2 cran.r-project.org/web/packages/t… @JanBroderEngler bioRxiv 2024 biorxiv.org/content/10.110…

@JLMart81 @DTUbioengineer Are these in our building?🤓

@yanrui_ye @Addgene Great to hear. Feel free to reach out if needed.

@tomas_strucko @Addgene It is brilliant to inactivate the KU70 gene transiently for Cas9-mediated genome editing. I am going to purchase these plasmids and try your genome-editing strategy. 🤔

The key plasmids from our Pichia tools paper academic.oup.com/femsyr/article… are now available @Addgene addgene.org/Uffe_Mortensen/ please share the news 🧑‍🔬🧬

The work from my short postdoc at @SMS_UWA @YitHengChooi lab is now published in Microbial Biotechnology @MicrobialBiote1 🧬🛠️🍄! We developed next-gen AMA1-plasmids for robust heterologous expression in filamentous fungi, great for genome mining (1/5)🧵 …icro-journals.onlinelibrary.wiley.com/doi/10.1111/17…

Our preprint "RoCi - A Single Step Multi-Copy Integration System Based on Rolling-Circle Replication" is online at @bioRxiv_synbio @biorxivpreprint doi.org/10.1101/2024.0…

Published today in @Nature, we describe an approach for single-molecule protein reading on @nanopore arrays. By utilizing ClpX unfoldase to ratchet proteins through a CsgG nanopore, we achieved single-amino-acid sensitivity. nature.com/articles/s4158…

Take a look at our paper about gene engineering tools for Komagataella phaffii published in FEMS #Yeast Reserach doi.org/10.1093/femsyr…. Thanks to all involved. @UHMortensenLab and @DTUbioengineer!

1/🧵 Better late than never! Excited to share my paper in PLOS Computational Biology: "teemi: An open-source literate programming approach for iterative design-build-test-learn cycles in bioengineering". Check it out! journals.plos.org/ploscompbiol/a…

Interested in ubiquitin and/or regulation of cell signalling in inflammation and metabolism? I’m happy to host applicants in my lab in Copenhagen🇩🇰, so just reach out to discuss potential project ideas 🧫🔬🧪🧑‍🔬👨‍🔬🥼