Daniel Kane

“...Time is the fire in which we burn.” -Delmore Schwartz 🪡 🧵 thread about #lactate oxidation and metabolic heat 🔥. /1 frontiersin.org/articles/10.33…

Wonderful to have played a small role in this effort. This century of biochemistry will place the staggering numeracy in the systems we study at the forefront of our science. Looking forward to more thinking like this👏 #Biochemistry #Redox #Bioenergetics

Here's the link to the paper. Download in the next 50 days for free! @ECU_Biology @ECUResearch @EastCarolinaDOI authors.elsevier.com/c/1iFnL5Sx5gtx…

@jwhitfie @brianglancylab @LBruceGladden Naked mole rats are awesome!

@mitokanedria @brianglancylab @LBruceGladden Thanks Dan - looking forward to diving in to these. Bonus points for the naked mole rat paper!

Lactate as an autocrine messenger? Lactate activates the mitochondrial electron transport chain independently of its metabolism. Would love to hear thoughts from @mitokanedria @brianglancylab @LBruceGladden sciencedirect.com/science/articl…

New publication from my lab about the metabolic (mitochondrial) effects of Yerba Mate (YM). We found disparate effects of YM in fat tissue and muscle tissue. In fat, YM increased metabolic rate (via greater mitochondrial uncoupling). However, in muscle, YM had no such effect--mitochondria were as efficient as ever. Overall, a uniquely beneficial series of changes that helps explain the general observations of YM facilitating weight loss and exercise performance. @dan_hues @Unicity mdpi.com/2072-6643/15/2…

@ChrisPerryMito @dhood_york @MHRC_YorkU Congratulations!! Excellent news!!

Through Dr. David Hood's leadership @dhood_york, the Muscle Health Research Centre @MHRC_YorkU has made great impact to scientists + trainees in Ontario and beyond. Together, we are forming the next phase for the Centre that will see new initiatives + partnerships. Stay tuned!

@Xin_Cai_ @UTSWNews @UTSW_RadOnc Interesting! Might the increased rate of oxygen consumption with lactate be due to its potential electrogenic accrual in mitos, as recently reported (pubmed.ncbi.nlm.nih.gov/36309010/)?

Happy to share a preprint of my postdoc work. Grateful to the many collaborators and supporters. Will continue this work in my lab @UTSWNews @UTSW_RadOnc Lactate activates the mitochondrial electron transport chain independent of its metabolism biorxiv.org/content/10.110…

Since the discovery of CRISPR-Cas systems, people have wondered if a similar type of system exists in eukaryotes. Today, we report in @Nature the characterization of Fanzor, a eukaryotic RNA-guided system that can be reprogrammed for human genome editing. nature.com/articles/s4158…

🔥thread alert: origins of the Crabtree effect

@lydfinley Thank you for this🔥thread! Do you think it would be fair(er) to say the Crabtree effect = inhibition by glycolysis, rather than by glucose? Experiments in which respiration is rescued by poisoning glycolysis (e.g., with iodoacetate) seem to support. #b0200" target="_blank" rel="nofollow noopener">sciencedirect.com/science/articl…

My❓: why is the Crabtree effect associated with yeast metabolism when Crabtree worked in mammalian cells? This review has the answer + historical perspective on the early studies teasing apart the relationship between oxygen, fermentation and respiration sciencedirect.com/science/articl…

Time for summer fun 🌞⛱️: let's talk about the Crabtree effect 🦀🦀, an ultimate #metabolismclassic, describing the ability of glucose to represses mitochondrial respiration ncbi.nlm.nih.gov/pmc/articles/P… 1/n

The pyruvate dehydrogenase complex: Life’s essential, vulnerable and druggable energy homeostat. By P.W. Stacpoole and C.E. McCall. 2023. 🔥 pubmed.ncbi.nlm.nih.gov/36863425/

@WeightInnovate Interesting! Perhaps related(?): rdcu.be/deSSq nature.com/articles/s4225…

“In conclusion, lactate is less effective than glucose and potentially detrimental during neural network rhythms featuring high energetic costs, likely through the lack of some obligatory ATP synthesis by aerobic glycolysis at excitatory and inhibitory synapses.”

@DrMarkBurnley No perfectly accurate terminology, but this seems quite close.

Ruh-roh! Concentration dependent?

Excited to announce our group's latest preprint led by @IlyaNBoykov + @Mcmontgomery11 biorxiv.org/content/10.110… In it we compared OXPHOS expression/function, across various murine tumor types, matched normal tissues and other organs -- all normalized to mito content. 🧵below

@oval_MetFlex And as if the plot weren’t already thick enough, there’s this: …iencejournals.onlinelibrary.wiley.com/doi/10.1002/mr…

@mitokanedria Good point. They performed a western blot on LDHA with no response from mito well and a lot from the cytosol well. Did not see anything on LDHB in paper. To be continued...

Anyone digested this paper re: FiLa? Technical (with cool images) yet relevant to discussions around lactate. "FiLa showed...not only that there was a lactate pool inside mito...but lacate level in mito signif higher than cytosol" Big news or whatevs? pubmed.ncbi.nlm.nih.gov/36309010/

@BobbyRaeburn Took me a second 😂

@mitokanedria I heard that the structure of Benzene came to Dr Benjamin Zene, a dream of tadpoles grabbing tails and swimming in a circle. 😁

The periodic table of elements came to Mendeleev in a dream?! themarginalian.org/2016/02/08/men…

A mechanistic link between the hyperglycemia of T2D and Alzheimer’s Disease involving KATP channels 👇