Sean Halper أُعيد تغريده
Very happy to announce the publication of Synthesis Success Calculator! Hearty congratulations to the fearless and amazing first author @halper_1! Paper: pubs.acs.org/doi/full/10.10…
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Sean Halper
30 posts
Sean Halper أُعيد تغريده
Super Hat Tip to @bioalgorithmist who worked tirelessly on this massive design problem w/o much sleep. As always, thanks to @alexcampreis for help with conception, protocol dev, and schematics and to @SarthokR for cross-strain validation.
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Sean Halper أُعيد تغريده
FOR IMMEDIATE RELEASE & USAGE: "A Massively Parallel COVID-19 Diagnostic Assay for Simultaneous Testing of 19200 Patient Samples" Protocol at docs.google.com/document/d/1kP…. Primers & Spike-in Controls at docs.google.com/spreadsheets/d…
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Sean Halper أُعيد تغريده
Interested in designing your own Extra Long sgRNA arrays? Check out their ELSA calculator here: salislab.net/software/desig… @hsalis Lab and @DeNovoDNA
Have a great #ResearchThuRsday and please reach out if you'd like to get featured yourself!
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Sean Halper أُعيد تغريده
Hat tip to @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist @phillip_clauer for their great work!
EBRC@EngBioRC
The @hsalis Lab at @Penn_State showed that simultaneous and stable coexpression of up to 28 single-guide RNAs can regulate 20+ genes w #CRISPR interference! Potential for reprogramming cells in many #SynBio #EngBio applications. nature.com/articles/s4158… @NatureBiotech
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Uh-oh - they've made an elephant *dung* gin. Notes of juniper, angelica, citrus, *earth*, and spice from the elephants’ favorite foraged botanicals. ibhu.co.za
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Sean Halper أُعيد تغريده
@PennStateChE @PennStateBME @BioinformPSU @hsalis @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist Science behind the scenes: Multiplexed CRISPR and sgRNA arrays with the @hsalis Lab #NBTintheNews via @NikoMcCarty @PLOSSynbio blogs.plos.org/synbio/2019/11…
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Sean Halper أُعيد تغريده
@NikoMcCarty just published a nice behind the scenes interview of my colleagues @alexcampreis, @halper_1 and my boss @hsalis on our recent ELSA paper. blogs.plos.org/synbio/2019/11…
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Sean Halper أُعيد تغريده
Sean Halper أُعيد تغريده
@PennStateChE @PennStateBME @BioinformPSU @hsalis @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist Co-authors @alexcampreis, @halper_1 & @hsalis go #behindthepaper to show how adding many-site targeting to CRISPR will have profound biotech implications go.nature.com/2IvU3YI
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Open access link to the ❄️ELSA❄️ paper for anyone stuck behind the paywall: nature.com/articles/s4158…
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Sean Halper أُعيد تغريده
Our work on Extra Long sgRNA Arrays (ELSAs) began during a free-wheeling graduate course. After designing several CRISPR-based circuits, we found many repetitive DNA sequences (a big #SynBio challenge). Learn more in this #NatureBiotech #BehindThePaper: bioengineeringcommunity.nature.com/users/320834-h…
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@alexcampreis double feature with the JLo CRISPR show(when that comes out)?
Amy Maxmen, PhD@amymaxmen
New Netflix series on #Crispr runs with the idea that anyone w/a pipette can make super humans in their garage 🙄. I've only seen the trailer but cringing #scicommgonewrong wired.com/story/netflixs…
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Sean Halper أُعيد تغريده
My new startup leverages CRISPR-Cas gene editing technology and proprietary machine learning algorithms to develop super long pumpkins (spookier).
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Sean Halper أُعيد تغريده
Sean Halper أُعيد تغريده
At a synthetic biology conference, a food truck handed out GMO papaya and yogurt samples to hundreds of attendees. “A lot of folks in our world aren’t using the term ‘GMO,’ and instead will say it’s something like ‘gene modification’ or ‘CRISPR’”
cnbc.com/2019/10/03/syn…
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Sean Halper أُعيد تغريده
Super useful for proteome and host engineering, its amazing how fast CRISPRi is moving #synbio
Howard Salis@hsalis
CRISPR is awesome, but if you stably co-express many sgRNAs, what could you do? You could: regulate many genes, edit many SNPs, redirect metabolism, & build cool circuits. Now introducing ELSAs to co-express 22 sgRNAs without introducing repetitive DNA. nature.com/articles/s4158…
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Sean Halper أُعيد تغريده
In a nutshell: Pick your targeted genes (up to 20). A designed ELSA regulates them (repression shown). Fully non-repetitive sequences ==> easy to synthesize & assemble. Genetically stable & evolutionary robust, great for creating selective (growth-inhibiting) phenotypes.
Nature Biotechnology@NatureBiotech
Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays go.nature.com/2IuqJSm
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Sean Halper أُعيد تغريده
@s_verkuijl I read this. I think one of the most interesting things is that they show is that Cas9 quickly can become limiting in the formation of active RNPs when there are competing guides around. Something we have suspected but never proved.
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