Sean Halper retweetledi
Very happy to announce the publication of Synthesis Success Calculator! Hearty congratulations to the fearless and amazing first author @halper_1! Paper: pubs.acs.org/doi/full/10.10…
English
Sean Halper
30 posts
Sean Halper retweetledi
Super Hat Tip to @bioalgorithmist who worked tirelessly on this massive design problem w/o much sleep. As always, thanks to @alexcampreis for help with conception, protocol dev, and schematics and to @SarthokR for cross-strain validation.
English
Sean Halper retweetledi
FOR IMMEDIATE RELEASE & USAGE: "A Massively Parallel COVID-19 Diagnostic Assay for Simultaneous Testing of 19200 Patient Samples" Protocol at docs.google.com/document/d/1kP…. Primers & Spike-in Controls at docs.google.com/spreadsheets/d…
English
Sean Halper retweetledi
Interested in designing your own Extra Long sgRNA arrays? Check out their ELSA calculator here: salislab.net/software/desig… @hsalis Lab and @DeNovoDNA
Have a great #ResearchThuRsday and please reach out if you'd like to get featured yourself!
English
Sean Halper retweetledi
Hat tip to @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist @phillip_clauer for their great work!
EBRC@EngBioRC
The @hsalis Lab at @Penn_State showed that simultaneous and stable coexpression of up to 28 single-guide RNAs can regulate 20+ genes w #CRISPR interference! Potential for reprogramming cells in many #SynBio #EngBio applications. nature.com/articles/s4158… @NatureBiotech
English
Uh-oh - they've made an elephant *dung* gin. Notes of juniper, angelica, citrus, *earth*, and spice from the elephants’ favorite foraged botanicals. ibhu.co.za
English
Sean Halper retweetledi
@PennStateChE @PennStateBME @BioinformPSU @hsalis @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist Science behind the scenes: Multiplexed CRISPR and sgRNA arrays with the @hsalis Lab #NBTintheNews via @NikoMcCarty @PLOSSynbio blogs.plos.org/synbio/2019/11…
English
Sean Halper retweetledi
@NikoMcCarty just published a nice behind the scenes interview of my colleagues @alexcampreis, @halper_1 and my boss @hsalis on our recent ELSA paper. blogs.plos.org/synbio/2019/11…
English
Sean Halper retweetledi
Sean Halper retweetledi
@PennStateChE @PennStateBME @BioinformPSU @hsalis @alexcampreis @halper_1 @grace_vezeau @DanielCetnar @bioalgorithmist Co-authors @alexcampreis, @halper_1 & @hsalis go #behindthepaper to show how adding many-site targeting to CRISPR will have profound biotech implications go.nature.com/2IvU3YI
English
Open access link to the ❄️ELSA❄️ paper for anyone stuck behind the paywall: nature.com/articles/s4158…
English
Sean Halper retweetledi
Our work on Extra Long sgRNA Arrays (ELSAs) began during a free-wheeling graduate course. After designing several CRISPR-based circuits, we found many repetitive DNA sequences (a big #SynBio challenge). Learn more in this #NatureBiotech #BehindThePaper: bioengineeringcommunity.nature.com/users/320834-h…
English
@alexcampreis double feature with the JLo CRISPR show(when that comes out)?
Amy Maxmen, PhD@amymaxmen
New Netflix series on #Crispr runs with the idea that anyone w/a pipette can make super humans in their garage 🙄. I've only seen the trailer but cringing #scicommgonewrong wired.com/story/netflixs…
English
Sean Halper retweetledi
My new startup leverages CRISPR-Cas gene editing technology and proprietary machine learning algorithms to develop super long pumpkins (spookier).
English
Sean Halper retweetledi
Sean Halper retweetledi
At a synthetic biology conference, a food truck handed out GMO papaya and yogurt samples to hundreds of attendees. “A lot of folks in our world aren’t using the term ‘GMO,’ and instead will say it’s something like ‘gene modification’ or ‘CRISPR’”
cnbc.com/2019/10/03/syn…
English
Sean Halper retweetledi
Super useful for proteome and host engineering, its amazing how fast CRISPRi is moving #synbio
Howard Salis@hsalis
CRISPR is awesome, but if you stably co-express many sgRNAs, what could you do? You could: regulate many genes, edit many SNPs, redirect metabolism, & build cool circuits. Now introducing ELSAs to co-express 22 sgRNAs without introducing repetitive DNA. nature.com/articles/s4158…
English
Sean Halper retweetledi
In a nutshell: Pick your targeted genes (up to 20). A designed ELSA regulates them (repression shown). Fully non-repetitive sequences ==> easy to synthesize & assemble. Genetically stable & evolutionary robust, great for creating selective (growth-inhibiting) phenotypes.
Nature Biotechnology@NatureBiotech
Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays go.nature.com/2IuqJSm
English
Sean Halper retweetledi
@s_verkuijl I read this. I think one of the most interesting things is that they show is that Cas9 quickly can become limiting in the formation of active RNPs when there are competing guides around. Something we have suspected but never proved.
English