santosh

@BenParkerLab @ItsBiniR @UCDProteomics @ProteomicsNews Thanks @BenParkerLab this is indeed a good option similar to protease resistant Streptavidin. I can modify the Arg and Lys residues in a human cell lysate, and use this to pre-coat the beads every time!!

@funtoshs @ItsBiniR @UCDProteomics @ProteomicsNews Ah, I see. Maybe you could simply dimethylate a lysate and use this to preclear? Simple protocol here (obviously just using ‘light’ for your application) nature.com/articles/nprot…

Is there any report in literature on passivating beads using a Lys/Arg-free protein for pull-down or IP-MS? #TeamMassSpec @ItsBiniR @UCDProteomics @ProteomicsNews

@BenParkerLab @ItsBiniR @UCDProteomics @ProteomicsNews Yes on similar lines, thanks. To coat the beads to prevent non-specific binding rather than interact with a tagged bait.

@funtoshs @ItsBiniR @UCDProteomics @ProteomicsNews Do you mean something like this: embopress.org/doi/full/10.15…

@ItsBiniR @UCDProteomics @ProteomicsNews Sorry my question was not precise. I was wondering if coating the beads with a protein that lacks Lys/Arg to prevent non-specific binding is an idea that has been tested?

@Deepak_ddb @plantpostdocs @Sunil_KumarKR @aribidopsis @msubrandizzilab @ParkerLab_MPIPZ @Billion_of_Gods Congratulations Deepak!!!

Thanks @plantpostdocs-@Sunil_KumarKR @aribidopsis for this award, am honoured to be part of this cohort. I look forward to working with fellow awardees & paying it forward. A special thanks to @msubrandizzilab & @ParkerLab_MPIPZ @Billion_of_Gods for their continuous mentoring

@ItsBiniR @UCDProteomics @ProteomicsNews Great. Thank you @ItsBiniR

@funtoshs @UCDProteomics @ProteomicsNews Been there.. Finding a correlation between protein and peptide estimation values is always a challenge.. As Brett mentioned the best solution is testing it on a run.. I always do a quick run with 1uL of the digest if the loading peptide conc is crucial..

Starting with 2 ug of protein for S-trap digestion, I end up with 15 ug of tryptic peptides as reported by Thermo BCA colorimetric peptide assay. Where am I going wrong? #TeamMassSpec @ItsBiniR @UCDProteomics @ProteomicsNews

@UCDProteomics @ItsBiniR @ProteomicsNews Got it!! Thank you @UCDProteomics

@funtoshs @ItsBiniR @ProteomicsNews For the Micro's? I personally would not do < 10 ug's & the thermo peptide assay, even the fluorescent one, can be a random number generator. If you want to use this low starting amt, skip the assay and just load 1/3rd - 1/2 and prey it works

@DemichevLab @lilley_ks @CamCProteomics Congratulations

Incredibly honoured to receive the EuPA award. Tremendous thanks to @lilley_ks and all amazing colleagues at the @CamCProteomics, the birthplace of DIA-NN, as well as my fantastic lab members, our collaborators and the proteomics community for all the support!

“I'd also like to acknowledge my students (…) they've gone on to do many great things. I'm particularly proud of the fact that one of my students fired Sam Altman.“ 😳🫡

New from our team @TheCrick, led by @GrantPellowe. Eukaryotes are very good at folding multidomain proteins. Here we give some insight into how the human ribosome helps with this, by tuning how nascent domains fold and dock. 1/n biorxiv.org/content/10.110…

@kdonovan1008 @biorxivpreprint @KheewoongBaek @fischerlab1 This is great, congratulations. Is the methods section missing or am I mistaken? Thanks.

We are happy to share our new preprint @biorxivpreprint "Unveiling the hidden interactome of CRBN molecular glues with chemoproteomics" led by @KheewoongBaek and Rebecca Metivier of @fischerlab1 biorxiv.org/content/10.110…

@slavov_n @broadinstitute @slavovLab @NUBioE1 Can you please share the link with me? Thank you.

Next Tue @ noon, I will present at the Broad Institute. Functional biological explorations with single-cell proteomics DM for link.

Our #FragPipe-Analyst manuscript is out! pubs.acs.org/doi/10.1021/ac…. Load FragPipe's TMT/ LFQ/DIA quant tables (peptide or protein quant) and get QC, Limma, PCA, volcano plots, heatmaps, do GO/pathway enrichment analysis, all with just a few clicks! Tutorials:fragpipe-analyst-doc.nesvilab.org

I wish journals would require methods papers be reproducible by at least 3 labs before it can be published . Think of all the time and resources this would save !

Eric Schmidt says in the next year, AI models will unite three key pillars: very large context windows, agents and text-to-action, and no-one understands what the impact will be but it will involve everyone having a fleet of AI agents at their command

@Sanjusinha7 Thank you for this thread.

This exercise certainly taught me that reading original nobel works and summarizing it is a task of pure joy. :) Here’s the original paper. Cheers! #gr2" target="_blank" rel="nofollow noopener">cell.com/cell/fulltext/…

Coming to Flaws: 1) The approach to remove one factor at a time is suboptimal. Will miss pairs that can buffer each other (OR gate). We also do not know how these four factors really induce this property (each of their job, steps, order, another nobel prize is here).

@bharath_IGC Wonderful...hearty congratulations Bharath

I am incredibly humbled to be accepted into the Fellowship of the Royal Society of Biology (RSB). This honour is a testament to the support & guidance of my colleagues, peers & mentors. I look forward to contributing to the advancement of the biological sciences & to inspire ECRs

New analysis from the @royalsociety shows just how high UK visa costs for researchers are compared to upfront costs for similar visa routes in 13 other countries. Here, we take a look at how the UK compares to countries in the G7 included in the analysis 🔍⤵️ 1/5