Gray Huffman

@edemmott Way to go, @edemmott!

Confirmed in appointment and a permanent contract (as close to tenured as we get in the uk)! 🍾🎉 Massive thanks to all the lab members, mentors, collaborators and colleagues over the years who made this this happen.

@roumenina @slavov_n @NUBioE1 @slavovLab Hi Professor Roumenina, thanks for your interest in attending this talk! I'll send you the Zoom link momentarily.

@slavov_n @GrayHuffman @NUBioE1 @slavovLab Hi, is it open for external audience? If yes, could you, please, send me the link?

Next Wednesday, @GrayHuffman will defend his dissertation. Join us if you are on campus (or on zoom)!

Head over to MaxQuant.live to try out the newest version of @TheCoxLab and @quiztoph's excellent software platform for targeted and prioritized data acquisition! It was a pleasure collaborating with you guys, as well, @quiztoph!

We always aimed to link single-cell protein measurements to cellular functions and regulatory mechanisms. This project was a major step for us in this directions, especially with the direct measurements proteolysis products: biorxiv.org/content/10.110… Data: scp.slavovlab.net/Huffman_et_al_…

@slavov_n @EastCoast55 @EastCoast55, thanks to @quiztoph and @TheCoxLab, prioritized analyses are no more difficult to set up than your standard MS method. Let me know if you'd like to set up a Zoom call to chat about the workflow

@EastCoast55 We will be happy to help if you have questions. @GrayHuffman has years of experience and knows all details from inside out and outside in.

This is a huge step forward and has a lot of applications beyond single cell work.

@Lo_Zanzi @Marco_Di_Gioia @NASPGHAN @CrohnsColitisFn Way to go, @Marco_Di_Gioia!

So proud of @Marco_Di_Gioia for his recognition by @NASPGHAN and @CrohnsColitisFn! More great things to come! Really very #proudPI

Did you think that data completeness of DIA could be improved?

I'm very excited to share the work we've done @slavovLab in collaboration with @DemichevLab! Increasing the throughput of sensitive proteomics by plexDIA biorxiv.org/content/10.110…

Yes, stay tuned ... more is coming, ... and it cannot be soon enough.

.@_lukekhoury and I highlight the identification of a signaling interactome and outline how MS proteomics can be combined with deep learning towards comprehensive identification of regulatory protein networks pubs.acs.org/doi/10.1021/ac…

@_AndrewLeduc's talk on nanoliter-scale single-cell sample prep just went up on YouTube. A great presentation for anyone interested in learning about the benefits of small-volume workflows! youtube.com/watch?v=ZiWIT2… #cellenONE #proteomics

Interested in evaluating your own single-cell proteomics data sets with a user friendly and well documented pipeline? Check out the scp R package and accompanying preprint from @c_vanderaa and @lgatt0: biorxiv.org/content/10.110… #SingleCell #Bioinformatics #proteomics

@KentsisResearch @slavovLab Thanks so much, @KentsisResearch; looking forward to catching your talk tomorrow afternoon!

Terrific talk by @GrayHuffman on the DO-MS program for visualizing MaxQuant results, including metrics for single-cell proteomics: terrific work! @slavovLab #SCP2019

Led by @harrisonspecht and just in time for #scp2019, our next-generation version of single cell proteomics: SCoPE2 is now out on bioRxiv! In it we apply SCoPE2 to the study of macrophage differentiation. twitter.com/biorxivpreprin…

Thanks for coming to my twitter poster! Development of click-amplifying FISH (clampFISH) probes to exponentially amplify RNA fluorescent signal in situ with single molecule resolution. All questions and comments welcome! @arjunrajlab #RSCPoster #RSCChemBio

Here's a preview of the DO-MS dashboard in action:

...whereas cross-cell-type peptide abundances are not. This type of plot can serve as a useful metric for determining the accuracy of quantitation, as well as for determining potential sample prep issues, such as poor label quenching (for which figure a. can also be useful).

If you're interested in optimizing your LC-MS/MS-driven #proteomics workflows, we hope you'll check out DO-MS, an opensource R-based dashboard for visualizing search results #RSCPoster #RSCChemBio #RSCAnalytical