Thiagarajah Lab

🧬 New preprint! First data from the Gut-AGE study: A comprehensive cellular & spatial atlas of the pediatric duodenum integrating single-cell and spatial profiling with diet, social drivers of health, and environmental exposures. biorxiv.org/cgi/content/sh…

@DrRosenIBD Thanks!

Congrats to your team. I look forward to reading it!

@Lo_Zanzi Thanks Ivan!

@JRTlab Many congrats!! Happy to see this out, fantastic story!

🚨 New lab paper ! 🚨 “A niche-dependent redox rheostat regulates epithelial stem cell fate.” An incredible amount of work spearheaded by a very talented scientist - Xi Chen! nature.com/articles/s4146…

@LizaKonnikova Thanks Liza!

@JRTlab Congrats Jay. What a great paper

@Synth_Immune See here! nature.com/articles/s4146…

@JRTlab Link?!

Intestinal stem cells live in niches that vary dramatically along the gut. But how do these microenvironments translate into changes in stem cell behavior? Our new work shows that cellular redox balance is a key integrator. 🧬⚡

Huge thanks to our collaborators and all the lab members who made this work possible! 🙌

Mechanistically, redox state creates a self-reinforcing circuit linking HIF1α signaling to post-translational regulation of IDH1, a key metabolic enzyme. This circuit couples local oxygen status to ISC metabolic programming. 🔄🧪

By tuning cellular redox balance, the niche can shift the balance between asymmetric ISC self-renewal and lineage commitment suggesting that redox is a decision-making signal for stem cells. 🌱⚖️

We found that hypoxia + Wnt signaling collaborate to restrict NOX1—a ROS-generating enzyme—to the crypt base in the distal colon. This spatial control shapes where stem cells maintain an oxidative state needed for cell-cycle entry. 🔥

We look at how intestinal stem cells integrate oxygen levels, Wnt signals, and metabolism for fate decisions in the unique environment of the distal colon

Also Michael’s paper on an optimized end-to-end system and method for multiplex immunofluorescence imaging - check it out : nature.com/articles/s4200…

A few lab updates: Our major consortium paper on the genetics of Congenital Diarrheas and Enteropathies came out last month. A major collab between BCH, SickKids (@AleixoMMuise), UCLA (Martin Martin) and Vanderbilt (@GoldenringLab ) pmc.ncbi.nlm.nih.gov/articles/PMC11…

We are recruiting postdoctoral fellows! Interested in using quantitative tools to study gene regulation in stem cell-derived developmental models? See below for more info and reach out

Super thrilled to share a preprint from the lab biorxiv.org/cgi/content/sh… We found pH regulates the inflammatory response 🔥in #macrophage by controlling BRD4-dependent transcriptional condensates. In my lab led by @younggouu

Modeling the cell biology of monogenetic intestinal epithelial disorders. New Review from Izumi Kaji (@iKajiLab), Jay Thiagarajah (@JRTlab), and James Goldenring (@GoldenringLab) @VUMChealth: hubs.la/Q02vqplj0 #Disease #Physiology #Trafficking

New postdoc opening in my lab in vascular remodeling studies. We do cool science. Email if interested. @atscommunity @atsearlycareer @endothelialnews @ATS_PC @vascularbiology @jfftweets @UMassVascSurg @PVRI

Pleasant tidings that our paper's recognized as one of @CellReports Best of 2023. Special congrats to @pascal_devant, @ElsyNgwa, & @BorsicElvira. Great collaborators @HaopengXiao & @echouchani. Super proud together with @JRTlab, @HafnerIva, & @jkagan1. cell.com/cell-reports/f…

@pascal_devant @jkagan1 @WuLabHarvard @Nature @harvardmed @BostonChildrens @VirologyHarvard @HarvardMicro Congrats Pascal - great study

Ever wondered how inflammatory caspases recognize IL-1 cytokines for cleavage? Read more in our new paper, a collaboration between @jkagan1 and @WuLabHarvard labs out today @Nature See🧵👇 1/ @harvardmed @BostonChildrens @VirologyHarvard @HarvardMicro nature.com/articles/s4158…