Manisha Kochar

Last week was a roller coaster- equal parts exhaustion & relief. After years of late nights, setbacks & small wins, I finally submitted my PhD thesis. Still letting it sink in… with gratitude, a little disbelief & a full heart✨ @IGIBSocial @foldingINvivo @kausikigib @dkumar02

@AggarwalAyush_ Congratulations Ayush!!

Ever wondered what happens at the molecular & cellular level when meningiomas invade the adjacent brain?Gene expression changes? More proliferation? Microenvironment interactions? Excited to share my first publication as a postdoc!! academic.oup.com/neuro-oncology…

New lab paper!! We develop a technology for live-cell, single-molecule visualization of proteasomal substrate degradation. We find that the site of substrate engagement by the proteasome determines decay kinetics, efficiency and co-factor requirement. biorxiv.org/content/10.648…

Pre-thesis colloquium ✔️ Presented my PhD work at CSIR-IGIB last month—grateful for the feedback and excited for the final stretch 🚀🧬 @foldingINvivo @IGIBSocial #PhDLife #AcademicTwitter #GradSchool #ProteinFolding

Mitochondrial presequences harbor variable strengths to maintain organellar function: rupress.org/jcb/article-ab…

Happy to share our latest work, out now in Molecular Cell! authors.elsevier.com/sd/article/S10…

A widespread protein misfolding mechanism is differentially rescued in vitro by chaperones based on gene essentiality: nature.com/articles/s4146…

Had a wonderful time earlier this month at the EMBO|India Lecture Series on “Structure, function and quality control of proteins in cell organelles” at @ccmb_csir. Great science, inspiring discussions, and a fun chance to explore the city as well. #EMBOorganelles #EMBOCCMB2025

How well do you think you understand chaperones? In our recent @MolSystBiol paper, @fried_lab's Divya Yadav shows that obligate chaperone clients in cells are totally different from the proteins that "need" chaperones during in vitro refolding. embopress.org/doi/full/10.10…

As the planet gets hotter & drier, can we make proteins & organisms more resilient to desiccation stress? Together with @shaharsu @SyracuseU & @alexholehouse @washumedicine, we show in @CellSystemsCP a sequence grammar encoding tolerance to dehydration. cell.com/cell-systems/a…

New online! The human ribosome modulates multidomain protein biogenesis by delaying cotranslational domain docking bit.ly/4gC2S2a

FROM CELL DEATH TO REGENERATION: What if dying cells -come back to life? Now online @embojournal I tinyurl.com/yp8m7he6 , we aim to answer this question. Here is the journey. If you🩷science, please dont 🛑till the end of 🧵, I promise you will not regret. 1/n

This EMBO practical course is one of the most comprehensive cryo-EM course. Please repost it and share with your colleagues. Deadline approaching soon! @EMBO, @iitdelhi, @unescorcb, @aiims_newdelhi, #CEM3DIP

Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC! guide.cryosparc.com/processing-dat…

Design of intrinsically disordered region binding proteins | Science science.org/doi/10.1126/sc…

Link here! guide.cryosparc.com/processing-dat…

We are delighted to share that @fried_lab's first work on neuroscience is now published in @ScienceAdvances. Using structural proteomics, we aim to dissect the molecular basis of cognitive decline using a rat model of cognitive aging. Short 🧵1/8 science.org/doi/10.1126/sc…

BioEmu generates protein conformational states at scale and predicts their equilibrium probabilities, providing insight into protein domain motions that could inform drug discovery science.org/doi/10.1126/sc… #NBThighlight

Targeted protein degradation in Escherichia coli using CLIPPERs | EMBO reports embopress.org/doi/full/10.10…

Just presented for the first time today by April @ProteinSociety who received their young investigator award! #ProudPI #FriedLabNews. Is Anfinsen’s Thermodynamic Hypothesis of protein folding true? We think PGK challenges it in a truly striking way. biorxiv.org/content/10.110…