Gabriel Barragan

The size of new DNA sequences that can be integrated into the human genome is a foundational constraint for engineering and enhancing human cells. In a new collaborative study, we’ve now almost doubled the maximum size of DNA sequences that can be efficiently inserted into primary human cells. biorxiv.org/content/10.648…

@sethbannon 🖐️

Goodies for 5050 gift boxes going out to labs. If you want one for your lab, let us know below!

@ScienceWansang Congrats!!! 🙌🙌🙌

Also, I am grateful for the Scholar-in-training award at #AACRIO2026! Many congratulations to the fellow awardees 👏

@fabian_theis Great work! What markers were used to define NKTs and MAIT cells in your dataset?

🚀 New preprint! The Human Cytokine Dictionary - 9.7M cells, 12 donors, 90 cytokines - our largest human single-cell perturbation atlas yet. Users can map cytokine activity in their own data. Huge thanks to Lukas, Sören, Larsen, Parse Bio & Seelig lab! 🔗 biorxiv.org/content/10.648…

@WeizmannScience I recently read his papers as I was writing my PhD thesis. Incredibly visionary work that established the foundation of the CAR-T field.

We, the Weizmann Institute of Science community, deeply mourn the passing of Prof. Zelig Eshhar of the Department of Immunology and Regenerative Biology. Prof. Eshhar was a trailblazing scientist in the field of cancer immunotherapy, a recipient of the Israel Prize in Life Science, and an acclaimed researcher who dedicated his life to life-saving research. May he rest in peace.

@ElowitzLab @andreww_lu @lkmoeller @Zhu_Lab @DJSiegwart I'm interested in circuit therapeutics and cell therapy! and have experience with some mouse models of cancers (albeit xenograft ones). Here is my preprint: biorxiv.org/content/10.110…

This work was led by brilliant students: @andreww_lu, @lkmoeller, and Stephen Moore, and is a collaboration with @Zhu_Lab and @DJSiegwart. If you have experience in animal cancer models and are interested in circuit therapeutics, please reach out.

Synthetic biology could enable new types of programmable therapeutics. Our new preprint introduces synthetic protein circuits that selectively trigger cell death in Ras-mutant cancer cells, with interesting advantages compared to existing approaches. biorxiv.org/content/10.110…

@seanm_x @pdhsu Nicholas Cage

We need a Grey’s Anatomy for the lab. People have no idea how much drama and gossip there is in research

@preprintclub Don't destroy me... Please 🥺 🥺 🥺. Just kidding fire away. Let me know if you have any comments or questions! 😃

In our Wednesday #DoubleFeatureEvent, Oxford’s own Tianming Zhao will present a #preprint from @Gab_brgn about the way IL-18 metabolically reprograms CAR-expressing natural killer T cells and enhances their antitumor activity against neuroblastoma. 🔞🚗🧠 biorxiv.org/content/10.110…

CD19-specific CAR T cells engineered to secrete interleukin-18 were used to treat patients with lymphoma after previous CAR T failure, with a complete or partial response of 81% and a complete response of 52%. Full trial results: nej.md/4jSiXkX

Ready for the general session #ASGCT2024 @asgc

@SabaGhassemi Thank you!

@Gab_brgn sigma does not carry it anymore. You have to get the four components and mix it. Unless they have a new product.

@SabaGhassemi Hi Saba, could you please share the catalog number of the deoxynucleosides you used in your paper? #Sec9" target="_blank" rel="nofollow noopener">nature.com/articles/s4155…

Refining chimeric antigen receptors via barcoded protein domain combination pooled screening #genetherapy #celltherapy dlvr.it/Sw31zC

@RossWilsonLab Congrats on your recent publication! I had a quick question regarding the methods; for the simultaneous KO experiments in figure 3, did you triple the amount of RNP (150pmol) or divide the 50pmol across the guides. Thanks!

GD2-CAR.15 NKTs are safe and can mediate objective responses in patients with NB. Additionally, their anti-tumor activity may be enhanced by targeting BTG1. @BCMCancerCenter @TexasChildrens nature.com/articles/s4159…

@MoKhalilLab @ScienceMagazine Great paper! Could the NS3 self cleaving protease domain result in an immune response?

Synthetic circuits that program new capabilities into human cells have tremendous potential to expand emerging gene and cell-based therapies. Our latest work, out today in @ScienceMagazine, reports a platform to accelerate these developments: (1/n) science.org/doi/10.1126/sc…

@plasmidsaurus any updates on setting up a courier service for Houston?