Nicholas Krasnow

17 posts

Nicholas Krasnow

Nicholas Krasnow

@NicholasKrasnow

Reshaping evolution with intelligent design | Chemistry PhD candidate @liugroup | @HarvardCCB @BroadInstitute | https://t.co/ILWS4Qvvkt

Katılım Ekim 2021
133 Takip Edilen112 Takipçiler
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Nicholas Krasnow
Nicholas Krasnow@NicholasKrasnow·
We’ve developed new prime editors by finding and addressing a stability bottleneck to gene editing. AI-redesigned reverse transcriptases give PE8 enhanced expression and activity across edit, delivery, and cell types. Congrats @Allentaoyz , @HoltSakai , @AllenYJiang , and co!
David R. Liu@davidrliu

Today in @NatureBiotech we report a new suit of PE8 prime editor proteins. PE8 variants were developed from laboratory-evolved PE6 proteins using AI-guided protein redesign. This approach combines recent advances in computational protein design and directed evolution to increase prime editing efficiency, especially in transient therapeutically relevant delivery settings such as mRNA+pegRNA electroporation into primary cells, eVLP delivery of prime editing RNPs, and LNP-mediated mRNA+pegRNA delivery in mice. drive.google.com/file/d/13VTcAs… 1/11

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Nicholas Krasnow
Nicholas Krasnow@NicholasKrasnow·
My colleagues @Sarah_E_Pierce and @stevenerwood show us how it’s now possible to address premature stop mutations that cause disease with a single precision gene edit, which enables the rescue of a variety of genes with one composition of matter.
David R. Liu@davidrliu

Today in @Nature we report a new prime editing strategy that can rescue a common cause of many genetic diseases in a disease-agnostic manner. This approach converts a redundant endogenous tRNA into an optimized suppressor tRNA, enabling a single prime edit to rescue premature stop codons across different diseases. (1/15) drive.google.com/file/d/1bSvkJW…

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Xin "Daniel" Gao
Xin "Daniel" Gao@danielxingao·
I'm thrilled to share that I’ve officially launched my lab this week at the University of Pittsburgh Department of Ophthalmology/UPMC Vision Institute @Pitt_OPH ! I’m incredibly grateful for the mentorship and support I’ve received throughout my journey from @davidrliu , @ESontheimerLab , and Chonghui Cheng during my postdoctoral and graduate training. I’ve also been very fortunate to collaborate with outstanding scientists like Joe Hacia, Cat Lutz, Nancy Braverman, and Job Dekker, whose partnership has been truly invaluable. A heartfelt thank you to all my past colleagues and those I’ve met during the faculty interview process. The mission of the Gao Lab is to uncover fundamental biological mechanisms and translate these discoveries into transformative therapies that advance human health. We are hiring at all levels! If you're passionate about driving the future of genomic medicine, I’d love to hear from you. Learn more about my work: lnkd.in/eZpAgYD9 and please reach out to me via danielgao@pitt.edu More updates coming soon—stay tuned!
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Allen Jiang
Allen Jiang@AllenYJiang·
I'm excited to share that I will be joining @UclaCBE as an Assistant Professor starting July 2027!
Allen Jiang tweet media
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Nicholas Krasnow
Nicholas Krasnow@NicholasKrasnow·
Check out this new development to gene-sized DNA integration technology, led by @Smriti__Pandey and @danielxingao in the Liu Lab. Their PACE-evolved and engineered recombinase enables precise, highly efficient insertion when coupled with prime editing in the eePASSIGE system.
David R. Liu@davidrliu

Today we report in @natBME the eePASSIGE system, which uses evolved and engineered recombinases and prime editing to integrate large gene-sized DNA cargoes into the mammalian genome in an efficient, precise, and targeted manner. (1/13) drive.google.com/file/d/1WDMVqL…

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Nicholas Krasnow
Nicholas Krasnow@NicholasKrasnow·
The latest generation cytosine base editors are out— evolved in PACE for high activity, base selectivity, and target sequence compatibility. Congrats, Emily and team!
David R. Liu@davidrliu

Today we report in @NatureComms the development of laboratory-evolved CBE6 cytosine base editors that offer high on-target C•G-to-T•A editing, virtually no A•T-to-G•C editing, low off-target editing, broad sequence context compatibility, and compatibility with multiple Cas domains. /1

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Nicholas Krasnow retweetledi
Cell Press
Cell Press@CellPressNews·
Stress rapidly increases your biological age, but this effect can be reversed if allowed time to recover. @DukeMPI Jame White, @gladyshev_lab Read more in @Cell_Metabolism
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Hugo Lowell
Hugo Lowell@hugolowell·
This has to be the craziest birthday present I have ever received, beating last year when we broke the story that Trump made calls on Jan. 6 using a White House phone that wasn’t reflected in the call log
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Ailong Ke's Lab at Yale MB&B
Ailong Ke's Lab at Yale MB&B@AilongK·
Caspases control programmed cell death in eukaryotes. Type III-E CRISPR harbors caspase-like proteases. Our new Science paper explains how Craspase switches on protease activity in RNA-guided fashion - safe alternatives to Cas nucleases in therapeutics. science.org/doi/10.1126/sc…
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Ailong Ke's Lab at Yale MB&B
Ailong Ke's Lab at Yale MB&B@AilongK·
IscB-ωRNA is as active as Cas9 in test tube, but only a fraction in size. Want to learn about their similarity and differences in high-res? What might be holding its activity back in human cells? Further miniaturization? Check out our new publication. science.org/doi/10.1126/sc…
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Keşfet

@Sarah_E_Pierce @stevenerwood @danielxingao @Pitt_OPH @davidrliu @ESontheimerLab @ScienceMagazine @SternbergLab