Shih-Feng You

Alzheimer’s disease does not begin with memory loss. It begins when your episodic memory network loses its ability to deactivate under load, driven by amyloid and tau disrupting synaptic signaling, which means your cognitive precision starts degrading years before you notice it. @NatureComms

Top medical journal posts a AI generated image as feature photo, it’s been three days and seems no one in @NEJM feel like to retract it. This showcases how much our academic and publication system paralyzed by AI.

To understand most genetic signals for human disease that are located in non-coding regions, we will need to explore their effects on gene expression at the single-cell level. Two new valuable resources apply this in brain tissue and neurological disease👇

Aging isn't just a dimmer switch; it’s a logistics failure. In @NatureAging, Doug Henze, Tony Wyss-Coray, and I show how mRNA "gets stuck" in aging microglia, causing degradation of their spatial context: nature.com/articles/s4358…

Distinct origins and niches determine the cellular responsiveness of CNS macrophages after repopulation nature.com/articles/s4159…

📢 Preprint: we present a whole-mouse-brain in vivo Perturb-seq atlas, 7.7 million cells, 1947 disease-associated perturbations, moving toward direct readout of how human genetics rewires cell states & circuits in vivo. Grateful for the Team! @NVIDIAHealth biorxiv.org/content/10.648…

Spatial perturb-seq: single-cell functional genomics within intact tissue architecture Scale up your in situ CRISPR screens Out in @NatureComms

Bioinformatics research assistant on WhatsApp github.com/Runchuan-BU/Bi…

geneSTRUCTURE: A Modern Platform for Visualization of Gene Structures biorxiv.org/content/10.648… 🧬💻🧪 github.com/hashimotoshump…

做生物、医学的朋友可以关注这个 skills：OpenClaw Medical Skills 震惊到，872个医学AI agent技能集全，从临床到生信、药物发现、医疗器械、健康管理一应俱全。 是时候让你的🦞学会医疗技巧了，一个🦞就是一个生物学信息医学信息专家： github.com/MedClaw-Org/Op…

#SpatialTranscriptomics Optimizing Xenium @10xGenomics In Situ data utility by quality assessment & best-practice analysis workflows If you are a Xenium user, this is a must-read🔥 👉Segmentation: Cellpose + Baysor (Segmentation-free alternative: SSAM, Points2Regions) 👉Preprocessing: Library size normalization, log-transformation, scaling, k-nearest neighbors graph (all PCs + 16 neighbors), Louvain clustering 👉Spatially variable feature selection: Squidpy Moran's I, Seurat Moran's I, Sinfonia, SomDE, Giotto, Hotspot 👉Imputation: SpaGE, Seurat, Tangram, SpaOTsc 👉Spatial domain identification: binning-based strategy, Bansky, SPACEL Performance vs CosMX, Molecular cartography, MERFISH, MERSCOPE, high-sensitivity ISS @naturemethods 2025 nature.com/articles/s4159…

PlotGDP: an AI Agent for Bioinformatics Plotting researchhub.com/paper/11075167…

A mouse circadian proteome atlas - it's a monster cell.com/molecular-cell…

PET tracers specific for activated and homeostatic microglia are in the works. One targets TREM2, the other P2Y12R. #alzheimersdisease @LMU_Muenchen ow.ly/kPJO50YcBGN

Clonal CD8+ T cells populate the leptomeninges and coordinate with immune cells in human degenerative brain diseases nature.com/articles/s4159…

@mvankerkhove @HHS_Jim @WHO As an epidemiologist, when you read in the email, “the cases have been isolated for treatment” and your conclusion is “they just here asking foe information”? Where you got your training?

All untrue @HHS_Jim. I know firsthand as #COVID19 technical lead: @WHO detected the Wuhan, China signal on 31 Dec 2019. Taiwan didn't warn us: they asked for information the same day. We have not ignored Taiwan, we don't ignore science and WHO never recommended lockdowns.

Cancer patients rarely get Alzheimer's. And a 15-year study in @Cell just explained why. They found a protein that clears brain plaques in mice - by activating the brain's own IMMUNE CELLS 🧵

A large study claims that in addition to ApoE4, ApoE3 is an Alzheimer’s risk allele, too. Without both of them, AD would be rare. ow.ly/B1Ou50Y20yq

Can antivirals against herpes treat early-stage Alzheimer's? At #AAIC2025, researchers presented data from a clinical trial showing no effect. My latest for @Being_Patient_ 1/6