Jon Daniels

@nc_znc @HillmanLab @naturemethods @LambertInst @ZuckermanBrain @venkatavoleti ASI has sub-licensed the relevant IP; please contact me if actually interested. Cost depends on what you need, e.g. the HiCam Fluo allows you to go super fast but adds a lot of cost over a more typical camera. Pretty safe to say between $100k and $400k.

@HillmanLab @naturemethods @LambertInst @ZuckermanBrain @venkatavoleti extremely cool! I'm doing a review of imaging systems for connectomics, and I was wondering whether you had any info about the approximate cost per imaging system with SCAPE 2.0? thank you!

Our @NatureMethods paper on SCAPE 2.0 is finally out! We paired #SCAPEMicroscopy with @LambertInst's #HiCAM to image the beating heart in 3D at over 300 volumes / second, tracked neurons in moving C. elegans worms + more! @ZuckermanBrain, @venkatavoleti, rdcu.be/bR25F

@astormic I'm not worried about getting galvos

@jsdaniel02 Do you have enough galvos?

Interested in getting imaging data like this? ASI is commercializing single-objective light sheet microscopes and might make one like DaXi if there are people who would buy it. DaXi originally developed by @loicaroyer and @Bin_YANG_Optics. vimeo.com/801341925

@RetoPaul @loicaroyer @Bin_YANG_Optics Let's say a "snouty-like" tertiary

@jsdaniel02 @loicaroyer @Bin_YANG_Optics Awesome! With a snouty tertiary?

DaXi is a SOLS design that simultaneously achieves sub-micron resolution (450nm laterally and 2um axially) and large FOV (volume of 3000 x  800 x 300 um with galvo, more with stage scanning).  Light sheet gentleness and speed.  Multi-view capability.  nature.com/articles/s4159…

@kmdouglass Thanks for sharing your thoughts! I too consider this paper to be the foundation for SOLS. I couldn't seem to figure out how to leave comments on the blog, but I could leave some here if that is preferred (I just don't visit this site often anymore).

Literature review: "An optical technique for remote focusing in microscopy," Botcherby et al., 2008 As far as I can tell, this paper provides the theoretical groundwork behind many of today's single objective light sheet microscopes. kylemdouglass.com/posts/literatu…

@AmirSTORMic @AndrewGYork @BaronDror @manorlaboratory I imagine details will be public no later than FOM with both a poster (by @AndrewGYork) and a talk (by @JamesDManton with @AndrewGYork on the author list).

@AndrewGYork @BaronDror @manorlaboratory And how do you determine this consistency in the data?

@xyzDrch @florianstroehl @carlasssmith (In my mind light sheet requires that the illumination propagation coincide with the detection focal plane.) But rather than debating terminology I'm more curious to understand how OLS and SOLIS and SOLEIL are fundamentally different.

@xyzDrch @florianstroehl It seems the optical concept behind both your papers plus "SOLEIL" by the lab of @carlasssmith is identical: oblique line illumination synchronized with camera's rolling shutter. None seem like light sheet but rather line confocal with oblique illumination. What am I missing?

What a start to 2024. Nobel Prize winner @Eric_Betzig and his team not only built a SOLIS system. No, they built 4! doi.org/10.1101/2023.1…

@kmdouglass I see no reason why this shouldn't work

I've got a 60x oil objective, and I don't care so much about the magnification change. I'm just wondering whether I can get a decent image across a ~100 um FOV or if the image will be significantly degraded by operating the tube lens outside its spec.

Anyone out there ever use a Nikon wide field tube lens with an Olympus objective in a telecentric configuration, i.e. objective and tube lens spaced by the sum of their focal lengths? I'm wondering whether I can use lenses that I already have on hand to do some BFP engineering.

@nvladimus @kmdouglass fair point, I was assuming a flat dichroic and considering transmission.

#microscopy folks: can anyone recommend a mount for a dichroic for a custom-built inverted microscope? The mount should be tip/tiltable, induce low stress on the dichroic, and be relatively light-tight. Related: do 3 mm dichroics induce noticeable astigmatism at high obj. NAs?

@kmdouglass Here is simulation with 3mm dichroic with 200mm f.l. tube lens with pupil corresponding to Nikon 60x/1.49 TIRF objective.

@kmdouglass Astigmatism if the dichroic is in focus space but none if in collimated space. In focus space, astigmatism scales linearly with glass thickness and the size of the back aperture and inverse linearly with lens focal length. "Noticeable" depends; can compare to diffraction limit.

@kmdouglass @kD3AN Yes indeed, that is the place.

@kD3AN @jsdaniel02 I think this was the site: davidlizarazo.pythonanywhere.com

@MinesBioImaging @AmirSTORMic Curious about the autocollimator, can you share how you made it and how you use it? I've heard of them before but never actually seen one in use.

@AmirSTORMic The #alignmentCrane and autocollimator are an absolute dream team. Would be absolutely crushed without it in terms of getting anywhere near aligned

My newest undergrad is getting really good at 3D printing. Now we can make all the things! Let the fun times begin

@lizarazojuan @loicaroyer @kmdouglass Hi David, that objective finder site is down again. If it's a battle to keep the URL active maybe there is some way to make public the underlying data you've gathered. I just care about being able to refer to it (which I do a few times a year). Thanks!

@jsdaniel02 @loicaroyer @kmdouglass Hey, Jon. I’ve fixed it!

Dear colleagues: Anyone has or know how to get Zemax blackbox models for Nikon Objectives? @NikonInst @NikonUSA @cbelthan

@ShahLabUCLA @microscopyEd Not yet, but at some point in the future I expect it to happen.

@microscopyEd @jsdaniel02 do you have alignment videos for this system like the great ones you made for the diSPIM?

@RetoPaul What happened really sucks. I hope you can somehow recover some of the costs. A real shame not to be able to share your cool developments due to multiple screw-ups outside your control.

@RetoPaul Fully agree that they should have called it earlier and got us all to hotels. Saying at 3am that we can get a hotel on them is silly. I had impression that the baggage made it to the 2nd plane but I was pretty groggy and concur that logistics fell apart.

@jsdaniel02 I have the feeling they did not plan the third attempt realistically. AFAIK, all luggage stayed in first plane, and that one could have been unloaded at 10pm, but wasn’t. After midnight, logistics apparently fell apart?

This is my first trip for a in person conference in over 3 years! So I am by no means a seasoned traveler. Is this nowadays normal that 3 planes don't work? And is it normal that airlines just let you fend for yourself when they give up? So far a nightmarish experience...