Jon Daniels

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Jon Daniels

Jon Daniels

@jsdaniel02

Scientific instrument developer, focused on #lightsheet and other #microscopy at @ASIImaging. 🔬 Rarely here; find me at https://t.co/4heTl2akuP

Eugene, OR Katılım Aralık 2016
419 Takip Edilen872 Takipçiler
Arya
Arya@aryamehr2552·
@jsdaniel02 Do you have enough galvos?
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Jon Daniels
Jon Daniels@jsdaniel02·
Interested in getting imaging data like this? ASI is commercializing single-objective light sheet microscopes and might make one like DaXi if there are people who would buy it. DaXi originally developed by @loicaroyer and @Bin_YANG_Optics. vimeo.com/801341925
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Jon Daniels
Jon Daniels@jsdaniel02·
DaXi is a SOLS design that simultaneously achieves sub-micron resolution (450nm laterally and 2um axially) and large FOV (volume of 3000 x  800 x 300 um with galvo, more with stage scanning).  Light sheet gentleness and speed.  Multi-view capability.  nature.com/articles/s4159…
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Jon Daniels
Jon Daniels@jsdaniel02·
@kmdouglass Thanks for sharing your thoughts! I too consider this paper to be the foundation for SOLS. I couldn't seem to figure out how to leave comments on the blog, but I could leave some here if that is preferred (I just don't visit this site often anymore).
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Kyle Douglass
Kyle Douglass@kmdouglass·
Literature review: "An optical technique for remote focusing in microscopy," Botcherby et al., 2008 As far as I can tell, this paper provides the theoretical groundwork behind many of today's single objective light sheet microscopes. kylemdouglass.com/posts/literatu…
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Jon Daniels
Jon Daniels@jsdaniel02·
@xyzDrch @florianstroehl @carlasssmith (In my mind light sheet requires that the illumination propagation coincide with the detection focal plane.) But rather than debating terminology I'm more curious to understand how OLS and SOLIS and SOLEIL are fundamentally different.
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Jon Daniels
Jon Daniels@jsdaniel02·
@xyzDrch @florianstroehl It seems the optical concept behind both your papers plus "SOLEIL" by the lab of @carlasssmith is identical: oblique line illumination synchronized with camera's rolling shutter. None seem like light sheet but rather line confocal with oblique illumination. What am I missing?
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Kyle Douglass
Kyle Douglass@kmdouglass·
I've got a 60x oil objective, and I don't care so much about the magnification change. I'm just wondering whether I can get a decent image across a ~100 um FOV or if the image will be significantly degraded by operating the tube lens outside its spec.
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Kyle Douglass
Kyle Douglass@kmdouglass·
Anyone out there ever use a Nikon wide field tube lens with an Olympus objective in a telecentric configuration, i.e. objective and tube lens spaced by the sum of their focal lengths? I'm wondering whether I can use lenses that I already have on hand to do some BFP engineering.
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Kyle Douglass
Kyle Douglass@kmdouglass·
#microscopy folks: can anyone recommend a mount for a dichroic for a custom-built inverted microscope? The mount should be tip/tiltable, induce low stress on the dichroic, and be relatively light-tight. Related: do 3 mm dichroics induce noticeable astigmatism at high obj. NAs?
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Jon Daniels
Jon Daniels@jsdaniel02·
@kmdouglass Here is simulation with 3mm dichroic with 200mm f.l. tube lens with pupil corresponding to Nikon 60x/1.49 TIRF objective.
Jon Daniels tweet media
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Jon Daniels
Jon Daniels@jsdaniel02·
@kmdouglass Astigmatism if the dichroic is in focus space but none if in collimated space. In focus space, astigmatism scales linearly with glass thickness and the size of the back aperture and inverse linearly with lens focal length. "Noticeable" depends; can compare to diffraction limit.
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Jon Daniels
Jon Daniels@jsdaniel02·
@MinesBioImaging @AmirSTORMic Curious about the autocollimator, can you share how you made it and how you use it? I've heard of them before but never actually seen one in use.
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Brandon Scott
Brandon Scott@MinesBioImaging·
@AmirSTORMic The #alignmentCrane and autocollimator are an absolute dream team. Would be absolutely crushed without it in terms of getting anywhere near aligned
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Brandon Scott
Brandon Scott@MinesBioImaging·
My newest undergrad is getting really good at 3D printing. Now we can make all the things! Let the fun times begin
Brandon Scott tweet media
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Jon Daniels
Jon Daniels@jsdaniel02·
@lizarazojuan @loicaroyer @kmdouglass Hi David, that objective finder site is down again. If it's a battle to keep the URL active maybe there is some way to make public the underlying data you've gathered. I just care about being able to refer to it (which I do a few times a year). Thanks!
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Jon Daniels
Jon Daniels@jsdaniel02·
@RetoPaul What happened really sucks. I hope you can somehow recover some of the costs. A real shame not to be able to share your cool developments due to multiple screw-ups outside your control.
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Jon Daniels
Jon Daniels@jsdaniel02·
@RetoPaul Fully agree that they should have called it earlier and got us all to hotels. Saying at 3am that we can get a hotel on them is silly. I had impression that the baggage made it to the 2nd plane but I was pretty groggy and concur that logistics fell apart.
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Reto Fiolka
Reto Fiolka@RetoPaul·
@jsdaniel02 I have the feeling they did not plan the third attempt realistically. AFAIK, all luggage stayed in first plane, and that one could have been unloaded at 10pm, but wasn’t. After midnight, logistics apparently fell apart?
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Reto Fiolka
Reto Fiolka@RetoPaul·
This is my first trip for a in person conference in over 3 years! So I am by no means a seasoned traveler. Is this nowadays normal that 3 planes don't work? And is it normal that airlines just let you fend for yourself when they give up? So far a nightmarish experience...
Reto Fiolka@RetoPaul

#FOM2023 @michelledigman and I are stranded in Newark, as the 3rd plane failed last night. I slept on chairs on the airport. Since @united made me check my carry on, and now cannot retrieve it, I will be for ~36h without new clothes. Anyone wants to host me in NYC for a talk?

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